Sachße (†), Dr. Hanns (H.S.)

These detection methods could be adapted to use in a clinical setting to direct subsequent patient treatment as well as in food safety and environmental assessment studies. E. fergusonii was also isolated from intestine, lung, liver, and kidney from a goat with a history of chronic diarrhea and emaciation, in Canada [4]. Thus, it is possible that these genes were transferred to E. fergusonii from Salmonella or E. coli, the species most closely genetically related to this bacterium (1), as reported for other genetic determinants (8). Eisenhaber, Dr. Frank (F.E.) A BLAST analysis of the Efer13 and EferYP products yielded E values of 4 × 10−117 for Efer13 and 0 for EferYP from E. fergusonii ATCC 35469. Sections of lung, liver, spleen, kidney, heart, rumen, abomasum, small, intestine, spiral colon, mammary gland, and brain were fixed in 10% buffered formalin, routinely processed for histology and examined. Escherichia fergusonii, the closest E. coli relative (Lawrence et al. Multiplex polymerase chain reaction for identification of Escherichia coli, Escherichia albertii and Escherichia fergusonii. Lechner-Ssymank, Brigitte (B.Le.) Some kinds of E. coli can cause diarrhea, while others cause urinary tract infections, respiratory illness and pneumonia, and other illnesses. The non-sorbitol-fermenting colorless colonies on SMA were kept frozen in TSB with 25% glycerol at −80°C for additional analysis. Jendrsczok, Dr. Christine (Ch.J.) Three of six samples that did not produce positive results by cultivation were positive by PCR. Despite treatments, the animal was found dead the next day and submitted for postmortem examination together with a blood sample drawn the previous day. Samples were then transferred to 10 ml of sterile buffered peptone water (EMD Chemicals, Gibbstown, NJ). Haug-Schnabel, PD Dr. Gabriele (G.H.-S.) The liver was tan/orange, slightly swollen with a marked reticular pattern. Multiple selective and differential medium types were used to first select presumptive isolates. In the present study, a negative reaction for sucrose in profiles J and K would lead to E. fergusonii results at 99.6% accuracy (profile B) and 99.8% accuracy (profile A), respectively (Table 5). Again, the DNA concentration was determined using the Invitrogen Qubit 2.0 Fluorometer. 2013, Article ID 829532, 3 pages, 2013. https://doi.org/10.1155/2013/829532, 1California Animal Health and Food Safety Laboratory System (CAHFS), Davis and Tulare Branches, 620 West Health Sciences Drive, Davis, CA 95616, USA. Hohl, Dr. Michael (M.H.) The BLAST analysis of all products from the putative Kluyvera isolate resulted in E. fergusonii as the only result with 100% coverage and an E value equivalent to zero. 2014 Aug 6;172(1-2):7-12. doi: 10.1016/j.vetmic.2014.04.016. Development and Assessment of a Single Tube Internally Controlled Multiplex PCR Assay to Detect Diff... Genome Sequences of Brucella abortus and Brucella suis Strains Isolated from Bovine in Zimbabwe. Hoos, Katrin (K.H.)  |  Here, E. fergusonii appears to be responsible for a severe, acute pneumonia and death in an adult cow. Determining the prevalence of food-borne pathogens such as Salmonella and Campylobacter in poultry production has been a topic of scientific concern for many years. Of more than 2,600 poultry farms, over 300 (12%) are located in the province of British Columbia (http://bcchicken.ca/wp-content/uploads/2012/11/BCChickenMarketingBoard-BOOKLETAnnualReport2012.pdf [accessed 4 July 2013]). Areas that had BLAST results of close to 100% coverage and an E value of 0 with respect to the finished genome of E. fergusonii ATCC 35469 (GenBank accession no. Dass die Entdeckerinnen von CRISPR-Cas9 den Preis irgendwann bekommen würden, war absehbar. Das könnte Sie auch interessieren: Spektrum der Wissenschaft Spezial Biologie – Medizin – Hirnforschung 4/2020 Bioethik, Spezial Biologie – Medizin – Hirnforschung 4/2020. Bogenrieder, Prof. Dr. Arno (A.B.) Grieß, Dr. Eike (E.G.) 2019 Mar;309(2):108-115. doi: 10.1016/j.ijmm.2018.12.003. Maheux AF(1), Boudreau DK, Bergeron MG, Rodriguez MJ. Means of the cycle threshold values and endpoint relative fluorescence units of 10-fold serially diluted DNA from E. fergusonii ATCC 35469a. Lange, Jörg  |  Within the necrotic areas, large numbers of Gram-negative coccobacilli were detected (Figure 2). Bacterial strains.The list of the 55 bacterial strains used in this study, including 11 reference strains from the American Type Culture Collection (ATCC; Cedarlane Laboratories, Burlington, Ontario, Canada), the previous characterized E. fergusonii ECD-227 strain (8, 12, 13), and an additional 43 field isolates from our collection, is presented in Table 1.

Seibt, Dr. Uta Phylogenetic tree, rooted on Escherichia fergusonii, of Achtman's sequence types (STs) encountered among 100 E. coli strains causing bacteremia in children aged <3 months. [wxyz], Anhäuser, Marcus (M.A.)

Kindt, Silvan (S.Ki.) This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This was particularly evident in the cranioventral region. For selection of presumptive E. fergusonii isolates, a loopful of each culture in TSB was first applied to Simmons citrate agar (Oxoid, Nepean, Ontario, Canada) containing 4% adonitol (SCA) as previously described (4). Lungs and liver were cultured on blood-agar and MacConkey-agar plates, incubated for 48 hours, 37°C in aerobic, CO2 enriched conditions (7% CO2). Both the conventional and real-time PCR assays described here are simple methods that could reduce the detection time of E. fergusonii from 6 days to 1. The supernatant was removed, and the pellet was resuspended in 200 μl of sterile water. Not only was this method more sensitive, specific and efficient, but also the processing was rapid and simple. Oehler, Prof. Dr. Jochen (J.Oe.)

The bands were referenced to a GeneRuler 100-bp DNA ladder (Fermentas, Ottawa, Ontario, Canada) and a 1-kb DNA ladder (New England BioLabs Inc., Whitby, Ontario, Canada) to size the amplicons. doi: 10.1128/IAI.00254-18. It is possible that E. fergusonii was present in these samples but was unable to compete with other gut bacteria during the enrichment step. Horn, Dagmar (D.H.) Sample collection and bacterial isolation.A total of 580 28-to-36-day-old broiler chickens were obtained from 32 farms in the Fraser Valley of British Columbia (Canada), a major area of poultry production. Colonization of the poultry gut by potential pathogenic bacteria such as E. fergusonii could result in the contamination of the environment and food chain. Detection by PCR can increase specificity while reducing the identification time. K. ascorbata is able to utilize citrate but not adonitol, leading to the formation of white colonies and the development of a blue color in the medium (20). The liver displayed centrilobular hepatocellular degeneration, with incipient hepatocyte necrosis (suggestive of hypoxic changes), mild histiocytic infiltration in sinusoids, and increased bile in canaliculi. Primers for amplification of Enterobacteriaceae, Enterococcus spp, Staphylococcus spp, Acinetobacterbaumanii and IC were designed and tested for sensitivity and specificity on the basis of their standard strains. Duell-Pfaff, Dr. Nixe (N.D.) The referring veterinarian treated the cow with IV fluids, Flunixin Meglumine, and Ceftiofur. Schön, Prof. Dr. Georg (G.S.) Epub 2014 May 9. Specificity testing on the candidate regions was performed using 30 E. coli isolates from each source. Conclusion: This new assay permits a rapid and accurate detection of some pathogenic microorganisms, that are among the most commonly detected ones in blood stream infections in Iran, with a simple and cost-effective method which includes the use of an internal control to validate the whole procedure thus avoiding false negative results. Although it is clearly possible that E. fergusonii can ferment MEL, none of the isolates with API 20E profiles G, H, and I provided positive results. Sie können unsere Newsletter jederzeit wieder abbestellen. Häbe, Martina (M.Hä.) Sander, Prof. Dr. Klaus (K.S.)

Bacterial numbers (CFU/ml) were determined in each dilution by viable counts on TSA. Significant intrafarm variations in prevalence were also noted within given regions (data not shown). J Bacteriol. HPV-Impfung | Frühe Impfung schützt deutlich vor Gebärmutterhalskrebs, Medizin-Nobelpreis 2020 | Spurensuche im Blut, Nobelpreis 2020 | Die Bekanntgabe des Medizin-Nobelpreises zum Nachschauen, Nobelpreis für Medizin | Auszeichnung für die Entdecker von Hepatitis C, Topsprinter | Weit vorne dank großem Hintern, Covid-19 | Mehr Totgeburten während der Pandemie, Allgemeinmedizin | Manche Hausärzte fühlen sich durch Leitlinien eingeschränkt, Nobelpreis für Physik 2020 | Am Ende der Zeit, Nobelpreis 2020 | Die Bekanntgabe des Physik-Nobelpreises zum Nachschauen, Nobelpreis für Physik 2020 | Entdeckung des Schwarzen Lochs im Milchstraßenzentrum ausgezeichnet, Physik | Forscher korrigieren Atomkernmasse, Ingenieurskunst | Laser machen Mikrorobotern Beine, Teilchenphysik | Das Higgs und die zweite Generation, Lügendetektoren | Der Traum von der Wahrheitsmaschine, Selbstbild | Worin wir uns selbst überschätzen. The sequences of Efer13 and EferYP PCR products from one such Kluyvera isolate and E. fergusonii ATCC 35469 were compared.